I-cell disruptor sisixhobo esisetyenziswa ngokuqhelekileyo esisetyenziselwa ukuqhekeza iiseli zebhayoloji kunye nokukhulula izinto ze-intracellular.Umgaqo wokusebenza we-cell breaker usekelwe kumgaqo wokuphulwa komzimba kunye ne-oscillation yomatshini, kwaye injongo yokuphulwa kweeseli ifezekiswa ngokunika amandla okwaneleyo ukutshabalalisa isakhiwo seeseli.
Umgaqo wokusebenza we-cell disruptor uya kwaziswa ngokubanzi ngezantsi.Amacandelo aphambili e-cell disruptor abandakanya umlawuli wesantya, igumbi lokutyumza, ibhola elityumzayo kunye nombhobho wesampula, njl. iisampuli kunye neebhola ezityumzayo, kunye neebhola ezityumzayo ziqhekeza iiseli ngokungqubana neesampuli.Ngaphambi kokusebenzisa i-disruptor cell, i-medium ephazamisayo efanelekileyo kufuneka ikhethwe kuqala.Imithombo yeendaba esetyenziswa ngokuqhelekileyo ngamaso eglasi, amaso esinyithi kunye namaso e-quartz.
Iingcamango eziphambili ekukhetheni i-medium yokutyumza yindalo yesampuli kunye nenjongo yokutyumza.Ngokomzekelo, kwiiseli ezibuthathaka, amaso eglasi amancinci angasetyenziselwa ukuphazamiseka;kwiiseli ezinzima ngakumbi, amaso esinyithi anzima anokukhethwa.Ngexesha lenkqubo yokutyumza, faka isampuli ukuba utyunyuzwe kwi-bin yokutyumza, kwaye ungeze inani elifanelekileyo lokutyumza okuphakathi.Emva koko, isantya sokujikeleza kwegumbi lokutyumza silawulwa ngumlawuli wesantya, ukwenzela ukuba i-medium yokutyumza kunye nesampuli ibe nokungqubana komatshini okuqhubekayo.Ezi zintlukwano zinokuphazamisa isakhiwo seseli ngokudluliswa kwamandla, ukuchithwa kweembrane zeseli kunye ne-organelles, kunye nokukhulula izinto ze-intracellular.
Inkqubo yokusebenza ye-cell disruptor ikakhulu ibandakanya ezi zinto zilandelayo eziphambili: isantya sokujikeleza, ubungakanani kunye nobuninzi bendlela yokutyumza, ixesha lokutyumza kunye nokushisa.Eyokuqala sisantya sokujikeleza.Ukukhethwa kwesantya sokujikeleza kufuneka kuhlengahlengiswe ngokweentlobo zeeseli ezahlukeneyo kunye neempawu zeesampuli.
Ngokuqhelekileyo, kwiiseli ezithambileyo, isantya esiphezulu sokujikeleza sinokukhethwa ukwandisa ukuphindaphinda kweengqungquthela kwaye ngaloo ndlela kuphazamise iiseli ngokufanelekileyo.Kwiiseli eziqinileyo, kuba ziqinile ngakumbi, isantya sokujikeleza sinokuncitshiswa ukunciphisa ukuphazamiseka kwesampulu.
Okwesibini ubukhulu kunye nobuninzi bendawo yokutyumza.Ubungakanani kunye nobuninzi be-medium yokutyumza buya kuchaphazela ngokuthe ngqo umphumo wokutyumza.Imithombo yeendaba ephazamisayo encinci inokubonelela ngamanqaku amaninzi okudibana, okwenza kube lula ukuphazamisa izakhiwo zeselula.Imidiya enkulu yokutyumza ifuna ixesha elide lokutyumza.
Ukongeza, ukuxinana kwendlela yokutyumza kuya kuchaphazela amandla ongquzulwano, ukuxinana okuphezulu kakhulu kunokukhokelela ekuqhekekeni okugqithisileyo kwesampulu.Ixesha lokuphazamiseka yiparameter ebalulekileyo yokuphazamiseka kweseli.Ukukhethwa kwexesha lokutyumza kufuneka kunqunywe ngokohlobo lwesampuli kunye nomphumo wokutyumza.Ngokuqhelekileyo, ixesha lokuphazamiseka lide, ngakumbi iiseli ziphazamiseka, kodwa kunokubangela umonakalo kwezinye iindawo zesampulu.Eyokugqibela kulawulo lobushushu.Umphumo wobushushu ekuqhekekeni kweeseli awunakungahoywa.Ukushisa okuphezulu kakhulu kunokubangela ukuchithwa kweeprotheni kunye ne-nucleic acids kwiiseli, ngaloo ndlela kuchaphazela umphumo wokuqhekeka.Ngoko ke, kucetyiswa ukwenza ukuphazamiseka kweeseli phantsi kweemeko ze-cryogenic, ezinokuncitshiswa ngokusebenzisa i-chiller okanye ukusebenza kwiqhwa.
Iziphazamisi zeeseli zidlala indima ebalulekileyo kuphando lwebhayoloji.Ngokulawula ngokufanelekileyo iiparitha ezifana nesantya sokujikeleza, ubungakanani kunye nobuninzi bexesha eliqhekezayo, ixesha lokutyumza kunye nobushushu, ukutyunyuzwa okusebenzayo kweeseli kunokufezekiswa.Emva kokuba iiseli ziphukile, iintlobo ezahlukeneyo zezinto kwiiseli zinokufumaneka, ezifana neeprotheni, i-nucleic acids, i-enzymes, njl., Ezibonelela ngesiseko esibalulekileyo sohlalutyo kunye nophando olulandelayo.Ngamafutshane, i-cell disruptor sisixhobo esibalulekileyo sokulinga, kwaye umgaqo wayo wokusebenza usekelwe kumgaqo wokuphulwa komzimba kunye nokungcangcazela komatshini.Ukuphazamiseka okusebenzayo kweeseli kunokufezekiswa ngokulawula iiparitha ezahlukeneyo ezifana nesantya sokujikeleza, ubungakanani kunye nobuninzi bokuphazamiseka okuphakathi, ixesha lokuphazamiseka kunye nokushisa.I-cell disruptor isetyenziswa ngokubanzi, ibonelela ngokulula kunye nenkxaso kubaphandi kuphando oluhambelanayo kwinkalo yebhayoloji.
Ixesha lokuposa: Sep-06-2023